Part:BBa_K2717008:Design

GFP-TGATG-mCherry

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1425
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 644
  

Design Notes

We adjusted the codon of the last lysine of GFP from AAA to AAG.

Source

GFP and mCherry is stored in our own lab.